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Comparaison de deux méthodes de prélèvement pour le dénombrement des larves de Crassostrea gigas en zone conchylicole ArchiMer
His, Edouard; Borel, Michel; Robert, Rene; Laborde, Jean-louis.
La numération des larves d'huître dans le plancton renseigne les ostréiculteurs sur la date de pose des collecteurs. La technique utilisée est très ancienne (BOURY, 1928) ; pour chaque station trois prélèvements par traits de filets pendant 10 minutes sont effectués : deux en surface (filet 200 et filet 130) et un à 1 mètre de profondeur (filet 130). Une méthode par pompage avec mesure instantanée du volume d'eau filtrée a été mise au point (prélèvements en surface et à 1 mètre de profondeur, et rétention des véligères sur filets 200). La comparaison des deux techniques met en évidence l'inadaptation de l'ancienne méthode. Le choix de la porosité (70 µm pour les filets 200) est mal adapté à la récolte des larves D (stade 1) dont le nombre peut être sous...
Tipo: Text Palavras-chave: Numération; Technique; Reproduction; Crassostrea gigas; Larves; Naissain; Recrutement; Ostréiculture.
Ano: 1987 URL: http://archimer.ifremer.fr/doc/1987/acte-6444.PDF
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Comparaison de la filtration entre une population de Crassostrea angulata Lmk et une population de Crassostrea gigas Thunberg élevées dans le bassin d'Arcachon ArchiMer
His, Edouard.
L'introduction d'une nouvelle espèce en ostréiculture donne souvent lieu à des débats passionnés dans les milieux ostréicoles. Un exemple est donné par Charles Martin, parqueur du Bassin d'Arcachon, dans son ouvrage "Agriculteur de la mer"; il rapporte les mesures restrictives qu'avait entraînées l'introduction de l'huître portugaise à Arcachon au début du 20ème siècle. Un des arguments avancés pour expliquer les réticences était celui de la "concurrence vitale" et de la compétition sur le plan nutritionnel (filtration) entre C. angulata et Ostrea edulis: cette dernière espèce était alors seule exploitée dans le bassin. Il fallut attendre l'épizootie de 1920, qui anéantit presque totalement l'huître plate, pour que la portugaise soit définitivement admise.
Tipo: Text Palavras-chave: Ostréiculture; Crassostrea gigas; Crassostrea angulata; Filtration; Bassin Arcachon; Gironde.
Ano: 1970 URL: http://archimer.ifremer.fr/doc/00009/11978/8682.pdf
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Comparaison de trois index de qualité de l'huître creuse Crassostrea gigas ArchiMer
Berthome, Jean-paul; Deslous-paoli, Jean-marc; Fernandez-castro, N..
Three indexes of quality of Japanese oyster Crassostrea gigas were studied in 3 lots of oysters bred in the r.1arennes-oléron basin from february to july 1979: condition index of MEDCOF &-. NEEDLER, thickness coefficient of IMAI & SAKAI and external quality coefficient of BERTHOME. These different indexes were correlated within each lot of oysters. The external quality coefficient is always significantly correlated with condition index, while thickness coefficient is correlated with other two only for one lot, but seemes useful to rate oyster growth better.
Tipo: Text Palavras-chave: Index de qualité; Croissance organisme; Crassostrea gigas; Huîtres.
Ano: 1979 URL: http://archimer.ifremer.fr/doc/1979/acte-2997.pdf
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Comparaison des performances d'élevage et de la vitalité de naissain d'huîtres creuses Crassostrea Gigas, en 3 sites du Morbihan (Rivière d'Auray et Baie de Quiberon), de mai 1999 à mars 2000 ArchiMer
Mazurie, Joseph; Fleury, Pierre-gildas; Claude, Serge; Hirata, Toshio; Langlade, Aime; Martin, Anne-genevieve; North, Benjamin.
Summer mortalities of young oysters have been studied in 1999 through the monitoring of a unique oyster batch distributed among 3 sites located from upstream of an estuary (Fort-Espagnol, Auray river) to downstream (Kerpenhir, Auray river) up to open bay (Men er Roué, Bay of Quiberon). Mortality and growth have been monitered in parallel with vitality indices, so as to try or identify periods of weakness of oysters. The spat grew from a mean weight of 3 g, in the beginning of May to around 35 g in November, without sharp differences between sites. The summer mortalities remained moderate (10 %) in the 2 most marine stations, whereas a light peak of mortality (15 %) was recorded in July in the most continental station. Any of the physico-chemical parameters...
Tipo: Text Palavras-chave: Huître creuse; Crassostrea gigas; Rivière d'Auray; Quiberon; Mortalité; Vitalité; Valvomètre; Force musculaire; Perte de sel; Cupped oyster; Spat; Gigas; Auray river; Of Quiberon; Mortality; Vitality; Valvometer; Muscle force; Salt loss; Hermeticity.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/00025/13632/10713.pdf
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Comparative analysis of oxygen consumption rates between cupped oyster spat of Crassostrea gigas of French, Japanese, Spanish and Taiwanese origins ArchiMer
Goulletquer, Philippe; Wolowicz, Maciej; Latala, Adam; Geairon, Philippe; Huvet, Arnaud; Boudry, Pierre.
Respiration rates of various geographical strains of Crassostrea gigas were compared to assess the respiratory expenditure as a physiological indicator of catabolism (cost of maintenance). Parental oysters, sampled in France (Marennes-Oleron), Japan (Hiroshima), Taiwan (Tunkang) and Spain (Cadix), were differentiated by both their geographic origin and by mitochondrial DNA markers, allowing the distinction between the two closely related taxa Crassostrea gigas and Crassostrea angulata. After reproduction of these parental oysters, respiration rates of spat of each strain, reared under common controlled conditions, were individually estimated at 20 degrees C by using a volumetric microrespirometer. Our results demonstrated that physiological variability...
Tipo: Text Palavras-chave: Pacific Ocean; NE Atlantic; Oyster strains; Hybrids; Crassostrea angulata; Crassostrea gigas; Metabolism; Respiration rates; Océan Pacifique; Atlantique NE; Souches génétiques; Hybrides; Crassostrea angulata; Crassostrea gigas; Métabolisme; Taux de respiration.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/publication-859.pdf
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Comparative histological study of gametogenesis in diploid and triploid pacific oysters (Crassostrea gigas) reared in an estuarine farming site in France during the 2003 heatwave ArchiMer
Normand, Julien; Le Pennec, Marcel; Boudry, Pierre.
We compared the temporal dynamics of gametogenesis in diploid and triploid Pacific oysters (Crassostrea gigas) using histology. Oysters were reared in an estuarine farming site in Brittany over 3 years and their gametogenesis was monitored over the exceptionally hot summer of 2003. Both diploids and triploids showed active gametogenesis, but a high proportion of triploids remained at early stages of gonad development. Gametogenesis of triploids was characterized by the simultaneous occurrence of gonias and immature cytes together with mature gametes rather than overall retardation, though some triploids showed complete gonad maturation. Evidence of spawning was seen in both groups, and the quantity of germinal products emitted appeared to be similar in...
Tipo: Text Palavras-chave: Triploidy; Crassostrea gigas; Oyster; Gametogenesis.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4152.pdf
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Comparative study of shell shape and muscle scar pigmentation in the closely related cupped oysters Crassostrea angulata, C. gigas and their reciprocal hybrids ArchiMer
Batista, Frederico; Ben-hamadou, Radhouan; Fonseca, Vera; Taris, Nicolas; Ruano, Francisco; Reis-henriques, Maria; Boudry, Pierre.
The taxonomic status of the cupped oysters Crassostrea angulata and C. gigas has received considerable attention in the last decades. Based on larval shell morphology, experimental hybridization, allozymes and nuclear DNA studies several authors have considered these two taxa as being synonymous. However, mitochondrial data showed clear genetic differences between the two taxa. In addition, microsatellite-based studies and cytogenetic studies have also provided evidence that supports their differentiation. Considerable differences have also been observed at the phenotypic level in terms of growth rate and ecophysiological parameters. In the present study, C. angulata from Sado estuary (Portugal) and C. gigas from Seudre estuary (France) were collected and...
Tipo: Text Palavras-chave: Crassostrea gigas; Crassostrea angulata; Hybridization; Shell pigmentation; Shell morphology.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-3904.pdf
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Comparison of haemocytic parameters among flat oyster Ostrea edulis stocks with different susceptibility to bonamiosis and the Pacific oyster Crassostrea gigas ArchiMer
Comesana, Pilar; Casas, Sandra M.; Cao, Asuncion; Abollo, Elvira; Arzul, Isabelle; Morga, Benjamin; Villalba, Antonio.
Farming of the flat oyster Ostrea edulis in Europe is severely constrained by the protozoan Bonamia ostreae. The introduction of the resistant species Crassostrea gigas has been a relief for the farmers, while the pilot programmes to select O. edulis strains resistant to bonamiosis performed in various countries can be seen as a promising strategy to minimise the effects of bonamiosis. However, the physiological bases of this differential susceptibility remain unknown. A search for an explanation of the intra and interspecific differences in oyster susceptibility to bonamiosis was accomplished by comparing some immune parameters among various O. edulis stocks and C gigas. On December 2003, naive and Bonamia-relatively resistant flat oysters from Ireland....
Tipo: Text Palavras-chave: Ostrea edulis; Crassostrea gigas; Bonamia ostreae; Haemocyte; Phagocytosis; Respiratory burst; Nitric oxide.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00074/18501/16092.pdf
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Comparison of hemocyte parameters in the pericardial cavity and the adductor muscle sinus in the Pacific oyster, Crassostrea gigas using two types of flow cytometers ArchiMer
Gagnaire, Beatrice; Duchemin, M; Auffret, Mathieu; Thomas-guyon, Helene; Renault, Tristan.
Parameters of hemocyte populations have been considered as relevant indicators of bivalve health and are currently used in immunotoxicological studies. Hemocytes in hemolymph can be collected by puncturing either the pericardial cavity or the adductor muscle sinus with a syringe. Flow cytometry is a methodological approach that is increasingly being used in laboratories for the study of hemocyte parameters in aquatic invertebrates. However, various protocols for hemocyte processing in laboratories equipped with different types of cytometers have been published. In this context, two flow cytometers ( EPICS XL4 (R), Beckman Coulter and FacsCalibur (R), Becton Dickinson) and two sites of hemocyte collection ( pericardial cavity and adductor muscle sinus) were...
Tipo: Text Palavras-chave: Crassostrea gigas; Phagocytosis; Hemocytes; Flow cytometry.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-3898.pdf
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Competition for food reduces disease susceptibility in a marine invertebrate ArchiMer
Pernet, Fabrice; Lugue, Klervi; Petton, Bruno.
Competition between organisms interfere in host and pathogen dynamics in ways that are difficult to predict. By one side, competitors can reduce the food supply and cause nutritional stress. Such stress could further modulate the susceptibility to infection by altering immune response or metabolic rate of the host. Alternatively, competitors may trap pathogens before they reach the focal host, and therefore reduce, enhance, or have no effect on infection according to the competitor's susceptibility to the infection. To better understand how competition influences host and pathogen interactions, we experimentally assessed the relative importance of competition for pathogens and resources on the severity of a viral disease infecting the Pacific oyster...
Tipo: Text Palavras-chave: Competition; Crassostrea gigas; Disease ecology; Filtration; Ostreid herpesvirus 1; Pathogen.
Ano: 2021 URL: https://archimer.ifremer.fr/doc/00692/80375/83493.pdf
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Composition de la matière organique particulaire dans les eaux du bassin de Marennes-Oléron : apport énergétique pour la nutrition de l'huître ArchiMer
Heral, Maurice; Razet, Daniel; Maestrini, Serge; Garnier, Jacqueline.
A twice monthly study shows that the major amount of organic material is from detrtic origin, and is brought in winter, when valeus of mineral seston are very high. In the sping time, phytoplancton blooms, do not make up for lack of detritic material where as in the summer, oceanic phytoplancton is composed of important amounts of carbohydrates. Available food is defined as sum of protids, lipids and carbohydrates and mesured in energetic therms. This allows to foretell the amount of food that molluscs ar likely to assimilate.
Tipo: Text Palavras-chave: Marennes Oléron; Nutrition; Matière organique; Crassostrea gigas; Huîtres.
Ano: 1980 URL: http://archimer.ifremer.fr/doc/1980/acte-3003.pdf
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Composition en éléments azotés minéraux et orgnaiques dissous de l'eau du bassin conchylicole de Marennes-Oléron ArchiMer
Heral, Maurice; Robert, Jean-michel; Truquet, Isabelle; Barbaroux, Olivier; Garnier, Jacqueline; Razet, Daniel.
Values of nitrogen nutrients (ammonia, nitrite and nitrate) are compared with values of total organic nitrogen and urea. In summer, the consumation by micro and macrophytes is added to less important telluric input of nineral nitroge. However, an important provision of organic nitrogen remains. On the contrary, in winter, organic nitrogen does not represent more than 10% of total nitrogen. The autors postulate that populations of oysters are at the starting point of the organic nitrogen production cycle.
Tipo: Text Palavras-chave: Crassostrea gigas; Biochimical composition; Oysters; Crassostrea gigas; Composition biochimique; Huîtres.
Ano: 1981 URL: http://archimer.ifremer.fr/doc/1981/acte-2940.pdf
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Compte rendu final du projet Triplofimer : Fiabilité et qualité de la production de naissains triploïdes d'huître creuse (Crassostrea gigas) ArchiMer
Suquet, Marc; Agogue, Julie; Brizard, Raphael; Haffray, Pierrick; Labbe, Catherine; Le Coz, Jean-rene; Pepin, Jean-francois; Rault, Paul; Saulnier, Denis.
Ce rapport fait état d'une étude réalisée au laboratoire de Génétique et Pathologie de l'IFREMER dans le cadre de l'analyse d'épisodes de mortalité anormale survenue en écloserie privée de crevettes pénéides. Plusieurs hypothèses ont été avancées. La présence de Vibrio harveyi a amené à constituer une collection de 40 souches à partir desquelles une identification bactériologique a été effectuée. Elle montre en effet une dominance de souches appartenant à un groupe homogène de Vibrio harveyi. La technique de ribotypage (digestion d'ADN génomique, transfert de Southem-blot et hybridation avec une sonde spécifique) est alors mise en place. Plusieurs difficultés techniques ont ralenti l'analyse et n'ont donc pas permis d'avoir des résultats assez avancés pour...
Tipo: Text Palavras-chave: Huître creuse; Crassostrea gigas; Tétraploïdes; Sperme; Conservation; Diagnostic zoo-sanitaire; Flore bactérienne; Herpès virus..
Ano: 2007 URL: http://archimer.ifremer.fr/doc/00304/41554/40762.pdf
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Compte rendu sur la reproduction des huîtres et des moules dans le bassin de Marennes Oléron pour l'année 1985 ArchiMer
Ratiskol, Jacqueline; Heurtebise, Serge.
Au cours de l'année 1985, le laboratoire CSRU de La Tremblade a pris en charge le suivi de la reproduction des larves d'huîtres et de moules dans le bassin de Marennes Oléron. Les différents points sont choisis en fonction de critères tels que la proximité des bancs géniteurs (gisements naturels d'huîtres ou de moules) et des zones traditionnelles de reproduction des huîtres : Les Doux et Mérignac pour le bassin, Faulx, Coux et Les Roches pour la Seudre, Les Palles et La mouclière pour la Charente.
Tipo: Text Palavras-chave: Reproduction; Larves; Crassostrea gigas; Mytilus edulis; Bassin de Marennes Oléron.
Ano: 1986 URL: http://archimer.ifremer.fr/doc/00049/16073/13534.pdf
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Compte rendu sur la reproduction des huîtres et des moules dans le bassin de Marennes Oléron pour l'année 1987 ArchiMer
Heurtebise, Serge; Ratiskol, Jacqueline.
Comme précédemment en 1985 et 1986, le suivi de la reproduction des huîtres et des moules a été assuré par le CSRU de La Tremblade pour les secteurs clefs de la production du naissain que sont l'embouchure de la Charente, la Seudre et le bassin de Marennes-Oléron. Ce suivi a nécessité 40 sorties en mer, dont 21 à partir du Chapus ou de La Tremblade (moyens nautiques IFREMER) et 19 au départ de Port des Barques avec la location des services d'un professionnel. Les différents paramètres physico-chimiques (T°, S %, 02) ont été mesurés à l'occasion de chaque pêche de larves pour appréhender au mieux l'évolution de la reproduction et fournir des indications complémentaires sur les bulletins de numération. Cette année, 30 bulletins sont parvenus à la profession...
Tipo: Text Palavras-chave: Reproduction; Larves; Crassostrea gigas; Mytilus edulis; Bassin de Marennes Oléron.
Ano: 1987 URL: http://archimer.ifremer.fr/doc/00049/16071/13533.pdf
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Concomitant herpes-like virus infections in hatchery-reared larvae and nursery-cultured spat Crassostrea gigas and Ostrea edulis ArchiMer
Renault, Tristan; Le Deuff, Rose-marie; Chollet, Bruno; Cochennec, Nathalie; Gerard, Andre.
Concomitant sporadic high mortalities were reported in France in May 1994 among batches of hatchery-reared larval Pacific oysters Crassostrea gigas and European flat oysters Ostrea edulis in 2 hatcheries, and in June and July 1994 among batches of cultured spat of both species in a shellfish nursery. Histological observation showed the presence of cellular abnormalities in moribund animals. Transmission electron microscopy revealed the presence of herpes-like virus particles in infected larvae and spat of both oyster species. This is the first description of a herpes-like virus infection in larval O.. edulis. Viruses observed in diseased larvae and spat of both species are similar with respect to ultrastructure and morphogenesis, They were detected...
Tipo: Text Palavras-chave: Apoptosis; Virus replication; Concomitant infection; Oyster mortality; Crassostrea gigas; Pacific oyster; Ostrea edulis; European flat oyster; Herpes like virus.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-820.pdf
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Conséquences génétiques de la production de larves d'huîtres en écloserie : étude des processus de dérive et de sélection ArchiMer
Taris, Nicolas; Sauvage, Christopher; Batista, Frederico; Baron, Sophie; Ernande, Bruno; Haffray, Pierrick; Boudry, Pierre.
Previous studies have shown heritable variation in larval developmental traits in the Pacific oyster Crassostrea gigas. In order to study the genetic consequences of production of oyster larvae in hatcheries, two factors, specific to hatcheries, were examined: the effect of discarding the smallest larvae (i.e. culling) and the effect of temperature (20°C versus 26°C). A mixed-family approach was used in order to infer the genetic composition of larval populations and family assignment, limiting possible environmental bias and allowing the study of a relatively large number of families using a limited number of larval tanks. Our results show that three multiplexed highly polymorphic microsatellite markers are a powerful tool for family assignment and,...
Tipo: Text Palavras-chave: Hatchery; Genetic diversity; Selection domestication; Larvae; Crassostrea gigas; Ecloserie; Diversité génétique; Sélection domestication; Larve; Crassostrea gigas.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-1505.pdf
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Conséquences génétiques de la production intensive de larves d'huîtres en écloserie : étude des processus de dérive et de sélection liés aux pratiques d'élevage. ArchiMer
Taris, Nicolas.
In order to study the genetic influence of intensive production of the larval stage in commercial hatcheries, two types of specific selective processes were examined: the effects of discarding the smallest larvae and temperature. A mixed-family approach was used in order to infer the genetic composition of the larvae. The results show that our mixed-family approach, combined with microsatellite-based family assignment, is a powerful tool for the study of bivalve larvae genetics. Selective sieving is an advantageous practice at a phenotypic scale, but also represents a substantial risk for diversity loss. The culled larvae are important for the minimization of the variability in reproductive success and the maximization of broodstock genetic diversity....
Tipo: Text Palavras-chave: Hatchery; Oyster; Genetic diversity; Selection domestication; Larvae; Crassostrea gigas; Ecloserie; Huître; Diversité génétique; Sélection domestication; Larve; Crassostrea gigas.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/these-976.pdf
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Contrat de Plan Etat Région Poitou-Charentes 1994-1998. Convention 95 RPC R 62 - Programme Pathologie - Rapport Scientifique année 1995 ArchiMer
Renault, Tristan; Berthe, Franck; Chollet, Bruno; Cochennec, Nathalie; Haffner, Philippe; Le Deuff, Rose-marie; Thuillier, Benoit.
Le but de ce programme pathologie est de rechercher et d'étudier les agents pathogènes connus et inconnus chez les mollusques bivalves marins, plus particulièrement chez l'huître creuse, Crassostrea gigas, afin d'identifier d'éventuels agents causant des mortalités, de proposer des mesures prophylactiques et de définir des conditions zootechniques permettant aux professionnels de limiter l'impact des maladies sur leur cheptel. La présence de maladies, chez les mollusques bivalves marins dont l'impact socio économique peut être catastrophique pour les productions conchylicoles, et l'accroissement des échanges de produits d'aquaculture entre les pays de la CEE et avec les pays tiers nécessitent le renforcement des recherches dans les domaines de la...
Tipo: Text Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae.
Ano: 1995 URL: http://archimer.ifremer.fr/doc/00043/15453/12828.pdf
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Contrat de Plan Etat Région Poitou-Charentes 1994-1998. Convention 96 RPC R70 - Programme Pathologie - Rapport Scientifique année 1996 ArchiMer
Renault, Tristan; Berthe, Franck; Chollet, Bruno; Cochennec, Nathalie; Haffner, Philippe; Le Deuff, Rose-marie; Lipart, Cecile; Thebault, Anne.
Le but de ce programme pathologie est de rechercher et d'étudier les agents pathogènes connus et inconnus chez les mollusques bivalves marins, plus particulièrement chez l'huître creuse, Crassostrea gigas, afin d'identifier d'éventuels agents causant des mortalités, de proposer des mesures prophylactiques et de définir des conditions zootechniques permettant aux professionnels de limiter l'impact des maladies sur leur cheptel. La présence de maladies, chez les mollusques bivalves marins dont l'impact socio économique peut être catastrophique pour les productions conchylicoles, et l'accroissement des échanges de produits d'aquaculture entre les pays de la CEE et avec les pays tiers nécessitent le renforcement des recherches dans les domaines de la...
Tipo: Text Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00043/15452/12827.pdf
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